Development of the TypeSeq Assay for Detection of 51 Human Papillomavirus Genotypes by Next-Generation Sequencing

We have developed a new human papillomavirus (HPV) genotyping assay for detection of 51 HPV genotypes by next-generation sequencing (NGS). The TypeSeq assay consists of 3 PCR steps that equalize viral load and each type’s amplicon copies prior to genotyping by NGS, thereby maximizing multiple-type s...

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Detalhes bibliográficos
Publicado no:J Clin Microbiol
Main Authors: Wagner, Sarah, Roberson, David, Boland, Joseph, Yeager, Meredith, Cullen, Michael, Mirabello, Lisa, Dunn, S. Terence, Walker, Joan, Zuna, Rosemary, Schiffman, Mark, Wentzensen, Nicolas
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2019
Assuntos:
Virology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6498001/
https://ncbi.nlm.nih.gov/pubmed/30814267
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.01794-18
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