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Direct real-time observation of actin filament branching mediated by Arp2/3 complex using total internal reflection fluorescence microscopy

Existing methods for studying actin filament dynamics have allowed analysis only of bulk samples or individual filaments after treatment with the drug phalloidin, which perturbs filament dynamics. Total internal reflection fluorescence microscopy with rhodamine-labeled actin allowed us to observe po...

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Autors principals: Amann, Kurt J., Pollard, Thomas D.
Format: Artigo
Idioma:Inglês
Publicat: The National Academy of Sciences 2001
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC64974/
https://ncbi.nlm.nih.gov/pubmed/11742068
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.211556398
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