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New procedure for epidermal cell isolation using kiwi fruit actinidin, and improved culture of melanocytes in the presence of leukaemia inhibitory factor and forskolin

OBJECTIVES: Conventional isolation of epidermis from the dermis and disruption of epidermal sheets to liberate the cells, are performed using proteolytic enzymes such as thermolysin or collagenase. Selective population expansion of melanocytes is achieved by suppressing proliferation of keratinocyte...

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Dades bibliogràfiques
Publicat a:Cell Prolif
Autors principals: Yarani, Reza, Mansouri, Kamran, Mohammadi‐Motlagh, Hamid Reza, Bakhtiari, Mitra, Mostafaie, Ali
Format: Artigo
Idioma:Inglês
Publicat: John Wiley and Sons Inc. 2013
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6496922/
https://ncbi.nlm.nih.gov/pubmed/23659789
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/cpr.12028
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