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Comparative analysis of widely used methods to remove nonfunctional myosin heads for the in vitro motility assay

The in vitro motility assay allows studies of muscle contraction through observation of actin filament propulsion by surface-adsorbed myosin motors or motor fragments isolated from muscle. A possible problem is that motility may be compromised by nonfunctional, “dead”, motors, obtained in the isolat...

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Detalhes bibliográficos
Publicado no:J Muscle Res Cell Motil
Main Authors: Rahman, Mohammad A., Salhotra, Aseem, Månsson, Alf
Formato: Artigo
Idioma:Inglês
Publicado em: Springer International Publishing 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6494787/
https://ncbi.nlm.nih.gov/pubmed/30850933
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10974-019-09505-1
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