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Live-cell imaging of human spermatozoa using structured illumination microscopy

Structural details of spermatozoa are interesting from the perspectives of fundamental biology and growing reproductive health problems. Studies of nanostructural details of these extremely motile cells have been limited to fixed cells, largely using electron microscopy. Here we provide the protocol...

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Detalhes bibliográficos
Publicado no:Biomed Opt Express
Main Authors: Opstad, Ida S., Popova, Daria A., Acharya, Ganesh, Basnet, Purusotam, Ahluwalia, Balpreet S.
Formato: Artigo
Idioma:Inglês
Publicado em: Optical Society of America 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6491005/
https://ncbi.nlm.nih.gov/pubmed/31065404
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/BOE.9.005939
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