Antigen-Specific Single B Cell Sorting and Monoclonal Antibody Cloning in Guinea Pigs

Here, we have established an antigen-specific single B cell sorting and monoclonal antibody (mAb) cloning platform for analyzing immunization- or viral infection-elicited antibody response at the clonal level in guinea pigs. We stained the peripheral blood mononuclear cells (PBMCs) from a guinea pig...

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Detalhes bibliográficos
Publicado no:Front Microbiol
Main Authors: Lei, Lin, Tran, Karen, Wang, Yimeng, Steinhardt, James J., Xiao, Yongli, Chiang, Chi-I, Wyatt, Richard T., Li, Yuxing
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2019
Assuntos:
Microbiology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6489837/
https://ncbi.nlm.nih.gov/pubmed/31065249
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2019.00672
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