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High-yield Production of Granulocyte-macrophage Colony-stimulating Factor in E. coli BL21 (DE3) By an Auto-induction Strategy

A novel strategy to increase protein expression yield is unintended induction of expression in complex media, called auto-induction. This method can be used to express proteins under control of the lac promoter without any need to monitor bacterial growth pattern, and addition of specific expression...

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Publicat a:Iran J Pharm Res
Autors principals: Malekian, Raziyeh, Jahanian-Najafabadi, Ali, Moazen, Fatemeh, Ghavimi, Reza, Mohammadi, Elmira, Akbari, Vajihe
Format: Artigo
Idioma:Inglês
Publicat: Shaheed Beheshti University of Medical Sciences 2019
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6487437/
https://ncbi.nlm.nih.gov/pubmed/31089381
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