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Identifying Unknown Enzyme-Substrate Pairs from the Cellular Milieu with Native Mass Spectrometry
The enzyme-substrate complex is inherently transient, rendering its detection difficult. In our framework, IsoLAIT (Isotope-Labeled, Activity-based Identification and Tracking), designed for bisubstrate systems, the common substrate, such as S-adenosyl-L-methionine (AdoMet) for methyltransferases, i...
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| Publicado no: | Chembiochem |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6483605/ https://ncbi.nlm.nih.gov/pubmed/28140508 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cbic.201600634 |
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