Antigen Extraction and B Cell Activation Enable Identification of Rare Membrane Antigen Specific Human B Cells

Determining antigen specificity is vital for understanding B cell biology and for producing human monoclonal antibodies. We describe here a powerful method for identifying B cells that recognize membrane antigens expressed on cells. The technique depends on two characteristics of the interaction bet...

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Udgivet i:Front Immunol
Main Authors: Zimmermann, Maria, Rose, Natalie, Lindner, John M., Kim, Hyein, Gonçalves, Ana Rita, Callegari, Ilaria, Syedbasha, Mohammedyaseen, Kaufmann, Lukas, Egli, Adrian, Lindberg, Raija L. P., Kappos, Ludwig, Traggiai, Elisabetta, Sanderson, Nicholas S. R., Derfuss, Tobias
Format: Artigo
Sprog:Inglês
Udgivet: Frontiers Media S.A. 2019
Fag:
Immunology
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6477023/
https://ncbi.nlm.nih.gov/pubmed/31040853
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fimmu.2019.00829
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