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Mass Spectrometry-Based Proteomics to Define Intracellular Collagen Interactomes
We present the development, optimization, and application of constructs, cell lines, covalent crosslinking methods, and immunoprecipitation strategies that enable robust and accurate determination of collagen interactomes via mass spectrometry-based proteomics. Using collagen type-I as an example, p...
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| Veröffentlicht in: | Methods Mol Biol |
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| Hauptverfasser: | , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2019
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6476175/ https://ncbi.nlm.nih.gov/pubmed/30840237 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-9095-5_7 |
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