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Purification of Myogenic Progenitors from Human Muscle Using Fluorescence-Activated Cell Sorting (FACS)

Primary myoblasts derived from human tissue are a valuable tool in research of muscle disease and pathophysiology. However, skeletal muscle biopsies, especially from diseased muscle, contain a plethora of non-myogenic cells, necessitating purification of the myogenic cell population. This protocol d...

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Bibliographic Details
Published in:Methods Mol Biol
Main Authors: Pakula, Anna, Spinazzola, Janelle M., Gussoni, Emanuela
Format: Artigo
Language:Inglês
Published: 2019
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6472699/
https://ncbi.nlm.nih.gov/pubmed/30367405
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-8897-6_1
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