Genotyping of Single Nucleotide Polymorphisms Using Allele-Specific qPCR Producing Amplicons of Small Sizes Directly from Crude Serum Isolated from Capillary Blood by a Hand-Powered Paper Centrifuge

The cell-free genomic DNA (gDNA) concentration in serum ranges from 1500 to 7500 copies/mL within 2 h after phlebotomy (6–24 times the concentration observed in plasma). Here, we aimed to evaluate the gDNA size distribution in serum with time after coagulation and to test if crude serum can be direc...

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Publicado en:Diagnostics (Basel)
Autores principales: Barra, Gustavo Barcelos, Santa Rita, Ticiane Henriques, Jardim, Daniella Paniago, Mesquita, Pedro Góes, Nobre, Camila Santos, Jácomo, Rafael Henriques, Abdalla Nery, Lídia Freire
Formato: Artigo
Lenguaje:Inglês
Publicado: MDPI 2019
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Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC6468353/
https://ncbi.nlm.nih.gov/pubmed/30641881
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/diagnostics9010009
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