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Import of amber and ochre suppressor tRNAs into mammalian cells: A general approach to site-specific insertion of amino acid analogues into proteins

A general approach to site-specific insertion of amino acid analogues into proteins in vivo would be the import into cells of a suppressor tRNA aminoacylated with the analogue of choice. The analogue would be inserted at any site in the protein specified by a stop codon in the mRNA. The only require...

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Autori principali: Köhrer, Caroline, Xie, Liang, Kellerer, Susanne, Varshney, Umesh, RajBhandary, Uttam L.
Natura: Artigo
Lingua:Inglês
Pubblicazione: The National Academy of Sciences 2001
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC64678/
https://ncbi.nlm.nih.gov/pubmed/11717406
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.251438898
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