A 16S rDNA PCR-based theoretical to actual delta approach on culturable mock communities revealed severe losses of diversity information

BACKGROUND: Subunits of ribosomal RNA genes (rDNAs) characterized by PCR-based protocols have been the proxy for studies in microbial taxonomy, phylogenetics, evolution and ecology. However, relevant factors have shown to interfere in the experimental outputs in a variety of systems. In this work, a...

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Dettagli Bibliografici
Pubblicato in:BMC Microbiol
Autori principali: dos Santos, Hellen Ribeiro Martins, Argolo, Caio Suzart, Argôlo-Filho, Ronaldo Costa, Loguercio, Leandro Lopes
Natura: Artigo
Lingua:Inglês
Pubblicazione: BioMed Central 2019
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Research Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC6454784/
https://ncbi.nlm.nih.gov/pubmed/30961521
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12866-019-1446-2
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