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Defining the RNA interactome by total RNA‐associated protein purification

The RNA binding proteome (RBPome) was previously investigated using UV crosslinking and purification of poly(A)‐associated proteins. However, most cellular transcripts are not polyadenylated. We therefore developed total RNA‐associated protein purification (TRAPP) based on 254 nm UV crosslinking and...

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Detalhes bibliográficos
Publicado no:Mol Syst Biol
Main Authors: Shchepachev, Vadim, Bresson, Stefan, Spanos, Christos, Petfalski, Elisabeth, Fischer, Lutz, Rappsilber, Juri, Tollervey, David
Formato: Artigo
Idioma:Inglês
Publicado em: John Wiley and Sons Inc. 2019
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6452921/
https://ncbi.nlm.nih.gov/pubmed/30962360
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.15252/msb.20188689
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