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CryoAPEX – an electron tomography tool for subcellular localization of membrane proteins
We describe a method, termed cryoAPEX, which couples chemical fixation and high-pressure freezing of cells with peroxidase tagging (APEX) to allow precise localization of membrane proteins in the context of a well-preserved subcellular membrane architecture. Further, cryoAPEX is compatible with elec...
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| Publicado no: | J Cell Sci |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The Company of Biologists Ltd
2019
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6451417/ https://ncbi.nlm.nih.gov/pubmed/30886003 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1242/jcs.222315 |
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