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Igni18, a novel metallo-hydrolase from the hyperthermophilic archaeon Ignicoccus hospitalis KIN4/I: cloning, expression, purification and X-ray analysis

The hyperthermophilic crenarchaeon Ignicoccus hospitalis KIN4/I possesses at least 35 putative genes encoding enzymes that belong to the α/β-hydrolase superfamily. One of those genes, the metallo-hydrolase-encoding igni18, was cloned and heterologously expressed in Pichia pastoris. The enzyme produc...

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Bibliographische Detailangaben
Veröffentlicht in:Acta Crystallogr F Struct Biol Commun
Hauptverfasser: Kobus, Stefanie, Perez-Garcia, Pablo, Hoeppner, Astrid, Holzscheck, Nicholas, Kovacic, Filip, Streit, Wolfgang R., Jaeger, Karl-Erich, Chow, Jennifer, Smits, Sander H. J.
Format: Artigo
Sprache:Inglês
Veröffentlicht: International Union of Crystallography 2019
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6450517/
https://ncbi.nlm.nih.gov/pubmed/30950832
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2053230X19002851
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