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Miniaturised interaction proteomics on a microfluidic platform with ultra-low input requirements

Essentially all cellular processes are orchestrated by protein-protein interactions (PPIs). In recent years, affinity purification coupled to mass spectrometry (AP-MS) has been the preferred method to identify cellular PPIs. Here we present a microfluidic-based AP-MS workflow, called on-chip AP-MS,...

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Detalhes bibliográficos
Publicado no:Nat Commun
Main Authors: Furlan, Cristina, Dirks, René A. M., Thomas, Peter C., Jones, Robert C., Wang, Jing, Lynch, Mark, Marks, Hendrik, Vermeulen, Michiel
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6449397/
https://ncbi.nlm.nih.gov/pubmed/30948724
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-019-09533-y
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