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EPR characterization of ascorbyl and sulfur dioxide anion radicals trapped during the reaction of bovine Cytochrome c Oxidase with molecular oxygen

The reaction intermediates of reduced bovine Cytochrome c Oxidase (CcO) were trapped following its reaction with oxygen at 50 μs to 6 msec by innovative freeze-quenching methods and studied by EPR. When the enzyme was reduced with either ascorbate or dithionite, distinct radicals were generated; X-b...

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Publicado en:J Magn Reson
Autores principales: Yu, Michelle A., Egawa, Tsuyoshi, Yeh, Syun-Ru, Rousseau, Denis L., Gerfen, Gary J.
Formato: Artigo
Lenguaje:Inglês
Publicado: 2009
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC6446898/
https://ncbi.nlm.nih.gov/pubmed/20056464
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmr.2009.12.017
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