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A Facile Method for the Removal of dsRNA Contaminant from In Vitro-Transcribed mRNA

The increasing importance of in vitro-transcribed (IVT) mRNA for synthesizing the encoded therapeutic protein in vivo demands the manufacturing of pure mRNA products. The major contaminant in the IVT mRNA is double-stranded RNA (dsRNA), a transcriptional by-product that can be removed only by burden...

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Detalhes bibliográficos
Publicado no:Mol Ther Nucleic Acids
Main Authors: Baiersdörfer, Markus, Boros, Gábor, Muramatsu, Hiromi, Mahiny, Azita, Vlatkovic, Irena, Sahin, Ugur, Karikó, Katalin
Formato: Artigo
Idioma:Inglês
Publicado em: American Society of Gene & Cell Therapy 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6444222/
https://ncbi.nlm.nih.gov/pubmed/30933724
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.omtn.2019.02.018
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