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Alpha protons as NMR probes in deuterated proteins
We describe a new labeling method that allows for full protonation at the backbone Hα position, maintaining protein side chains with a high level of deuteration. We refer to the method as alpha proton exchange by transamination (α-PET) since it relies on transaminase activity demonstrated here using...
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| Pubblicato in: | J Biomol NMR |
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| Autori principali: | , , , , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Springer Netherlands
2019
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6441447/ https://ncbi.nlm.nih.gov/pubmed/30762170 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10858-019-00230-y |
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