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An In Vitro Assay for Outer Membrane Protein Assembly by the BAM Complex

To elucidate the mechanism of a biochemical process it is often essential to reconstitute the reaction in vitro using the minimal set of factors required to drive the reaction to completion. Here, we describe a method to reconstitute the folding and membrane integration of bacterial outer membrane (...

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Detalhes bibliográficos
Publicado no:Methods Mol Biol
Main Authors: Roman-Hernandez, Giselle, Bernstein, Harris D.
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6440698/
https://ncbi.nlm.nih.gov/pubmed/26427687
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-2871-2_16
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