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Protein Micropatterning Assay - Quantitative analysis of protein-protein interactions

Characterization, especially quantification, of protein interactions in live cells is usually not an easy endeavor. Here, we describe a straightforward method to identify and quantify the interaction of a membrane protein (“bait”) and a fluorescently labeled interaction partner (“prey”) (membrane-bo...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Methods Mol Biol
Prif Awduron: Sevcsik, Eva, Weghuber, Julian, Lanzerstorfer, Peter, Schütz, Gerhard J.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: 2017
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC6436724/
https://ncbi.nlm.nih.gov/pubmed/28188535
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-6747-6_18
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