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Optimization of DamID for use in primary cultures of mouse hepatocytes

DamID, a method to identify DNA associating with a particular protein, was originally developed for use in immortalized tissue culture lines. The power of this technique has led to its adaptation for a number of additional systems. Here we report adaptations for its use in primary cells isolated fro...

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Bibliographic Details
Published in:Methods
Main Authors: Gatticchi, Leonardo, de las Heras, Jose I., Roberti, Rita, Schirmer, Eric C.
Format: Artigo
Language:Inglês
Published: Academic Press 2019
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6426339/
https://ncbi.nlm.nih.gov/pubmed/30445179
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2018.11.005
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