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A practical method for efficient and optimal production of Seleno‐methionine‐labeled recombinant protein complexes in the insect cells
The use of Seleno‐methionine (SeMet) incorporated protein crystals for single or multi‐wavelength anomalous diffraction (SAD or MAD) to facilitate phasing has become almost synonymous with modern X‐ray crystallography. The anomalous signals from SeMets can be used for phasing as well as sequence mar...
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| Veröffentlicht in: | Protein Sci |
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| Hauptverfasser: | , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
John Wiley & Sons, Inc.
2019
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6423997/ https://ncbi.nlm.nih.gov/pubmed/30663186 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.3575 |
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