Rapid and Simple Universal Escherichia coli Genotyping Method Based on Multiple-Locus Variable-Number Tandem-Repeat Analysis Using Single-Tube Multiplex PCR and Standard Gel Electrophoresis

We developed a multiplex PCR method based on multiple-locus variable-number tandem-repeat (VNTR) analysis (MLVA) that was designed for the rapid typing of Escherichia coli and Shigella isolates. The method amplifies seven VNTRs and does not require a sequencing capillary or fluorescent dyes. The amp...

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Publicado en:Appl Environ Microbiol
Autores principales: Caméléna, François, Birgy, André, Smail, Yasmine, Courroux, Céline, Mariani-Kurkdjian, Patricia, Le Hello, Simon, Bonacorsi, Stéphane, Bidet, Philippe
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Microbiology 2019
Materias:
Methods
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC6414366/
https://ncbi.nlm.nih.gov/pubmed/30610078
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02812-18
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