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Hydrogel droplet single-cell processing: DNA purification, handling, release, and on-chip linearization

The preparation and handling of mammalian single-cell genomic DNA is limited by the complexity bottleneck inherent to performing multi-step, multi-reagent operations in a microfluidic environment. We have developed a method for benchtop preparation of high-molecular weight, intact, single-cell genom...

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Publicat a:Biomicrofluidics
Autors principals: Zimny, Philip, Juncker, David, Reisner, Walter
Format: Artigo
Idioma:Inglês
Publicat: AIP Publishing LLC 2018
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6404942/
https://ncbi.nlm.nih.gov/pubmed/30867855
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1063/1.5020571
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