Split green fluorescent protein as a tool to study infection with a plant pathogen, Cauliflower mosaic virus

The split GFP technique is based on the auto-assembly of GFP when two polypeptides–GFP1-10 (residues 1–214; the detector) and GFP11 (residues 215–230; the tag)–both non-fluorescing on their own, associate spontaneously to form a fluorescent molecule. We evaluated this technique for its efficacy in c...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Dáder, Beatriz, Burckbuchler, Myriam, Macia, Jean-Luc, Alcon, Carine, Curie, Catherine, Gargani, Daniel, Zhou, Jaclyn S., Ng, James C. K., Brault, Véronique, Drucker, Martin
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2019
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Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6402836/
https://ncbi.nlm.nih.gov/pubmed/30840696
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0213087
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