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Evaluation of detection probabilities at the water-filtering and initial PCR steps in environmental DNA metabarcoding using a multispecies site occupancy model

Environmental DNA (eDNA) metabarcoding is a recently developed method to assess biodiversity based on a high-throughput parallel DNA sequencing applied to DNA present in the ecosystem. Although eDNA metabarcoding enables a rapid assessment of biodiversity, it is prone to species detection errors tha...

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Bibliografische gegevens
Gepubliceerd in:Sci Rep
Hoofdauteurs: Doi, Hideyuki, Fukaya, Keiichi, Oka, Shin-ichiro, Sato, Keiichi, Kondoh, Michio, Miya, Masaki
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Nature Publishing Group UK 2019
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6401178/
https://ncbi.nlm.nih.gov/pubmed/30837589
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-40233-1
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