Purification of cross-linked RNA-protein complexes by phenol-toluol extraction

Recent methodological advances allowed the identification of an increasing number of RNA-binding proteins (RBPs) and their RNA-binding sites. Most of those methods rely, however, on capturing proteins associated to polyadenylated RNAs which neglects RBPs bound to non-adenylate RNA classes (tRNA, rRN...

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Gepubliceerd in:Nat Commun
Hoofdauteurs: Urdaneta, Erika C., Vieira-Vieira, Carlos H., Hick, Timon, Wessels, Hans-Herrmann, Figini, Davide, Moschall, Rebecca, Medenbach, Jan, Ohler, Uwe, Granneman, Sander, Selbach, Matthias, Beckmann, Benedikt M.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Nature Publishing Group UK 2019
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Article
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6397201/
https://ncbi.nlm.nih.gov/pubmed/30824702
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-019-08942-3
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