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Nuclear import of Xenopus egg extract components into cultured cells for reprogramming purposes: a case study on goldfish fin cells

Reprogramming of cultured cells using Xenopus egg extract involves controlling four major steps: plasma membrane permeabilization, egg factors import into the nucleus, membrane resealing, and cell proliferation. Using propidium iodide to assess plasma membrane permeability, we established that 90% o...

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Bibliografski detalji
Izdano u:Sci Rep
Glavni autori: Chênais, Nathalie, Lorca, Thierry, Morin, Nathalie, Guillet, Brigitte, Rime, Hélène, Le Bail, Pierre-Yves, Labbé, Catherine
Format: Artigo
Jezik:Inglês
Izdano: Nature Publishing Group UK 2019
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6393519/
https://ncbi.nlm.nih.gov/pubmed/30814557
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-39500-y
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