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Optimization of an In Vitro Transcription/Translation System Based on Sulfolobus solfataricus Cell Lysate
A system is described which permits the efficient synthesis of proteins in vitro at high temperature. It is based on the use of an unfractionated cell lysate (S30) from Sulfolobus solfataricus previously well characterized in our laboratory for translation of pretranscribed mRNAs, and now adapted to...
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| Publicado en: | Archaea |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Hindawi
2019
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6388310/ https://ncbi.nlm.nih.gov/pubmed/30886540 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/2019/9848253 |
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