An efficient method for markerless mutant generation by allelic exchange in Clostridium acetobutylicum and Clostridium saccharobutylicum using suicide vectors

BACKGROUND: Clostridium acetobutylicum and Clostridium saccharobutylicum are Gram-positive, spore-forming, anaerobic bacterium capable of converting various sugars and polysaccharides into solvents (acetone, butanol, and ethanol). The sequencing of their genomes has prompted new approaches to geneti...

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Publicat a:Biotechnol Biofuels
Autors principals: Foulquier, Celine, Huang, Ching-Ning, Nguyen, Ngoc-Phuong-Thao, Thiel, Axel, Wilding-Steel, Tom, Soula, Julie, Yoo, Minyeong, Ehrenreich, Armin, Meynial-Salles, Isabelle, Liebl, Wolfgang, Soucaille, Philippe
Format: Artigo
Idioma:Inglês
Publicat: BioMed Central 2019
Matèries:
Research
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6375146/
https://ncbi.nlm.nih.gov/pubmed/30809274
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13068-019-1364-4
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