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Immunofluorescence Tomography: High-resolution 3-D reconstruction by serial-sectioning of methacrylate embedded tissues and alignment of 2-D immunofluorescence images

Immunofluorescence tomography is a high-resolution 3-D reconstruction method based on methacrylate embedding and serial-sectioning, where 2-D images of immuno-stained serial-sections are computationally aligned into image stacks, and the 3-D volume rendered. Butyl-Methyl Methacrylate (BMMA) plastic...

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Detalles Bibliográficos
Publicado en:Sci Rep
Autor Principal: Parfitt, Geraint J.
Formato: Artigo
Idioma:Inglês
Publicado: Nature Publishing Group UK 2019
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC6374383/
https://ncbi.nlm.nih.gov/pubmed/30760855
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-38232-9
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