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LiBiNorm: an htseq-count analogue with improved normalisation of Smart-seq2 data and library preparation diagnostics

Protocols for preparing RNA sequencing (RNA-seq) libraries, most prominently “Smart-seq” variations, introduce global biases that can have a significant impact on the quantification of gene expression levels. This global bias can lead to drastic over- or under-representation of RNA in non-linear len...

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Bibliografiske detaljer
Udgivet i:PeerJ
Main Authors: Dyer, Nigel P., Shahrezaei, Vahid, Hebenstreit, Daniel
Format: Artigo
Sprog:Inglês
Udgivet: PeerJ Inc. 2019
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6366399/
https://ncbi.nlm.nih.gov/pubmed/30740268
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7717/peerj.6222
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