Identification of reference genes for real-time PCR cytokine gene expression studies in sheep experimentally infected with Fasciola hepatica

The aim of this study was to validate reference genes for gene normalisation using qRT-PCR in hepatic lymph nodes (HLN) and livers from sheep infected with Fasciola hepatica during early and late stages of infection. To this end, a comprehensive statistical approach (RefFinder) encompassing four dif...

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Vydáno v:Sci Rep
Hlavní autoři: Pacheco, I. L., Abril, N., Zafra, R., Morales-Prieto, N., Hernández, V. Molina, Ruiz, M. T., Perez-Caballero, R., Martínez-Moreno, A., Pérez, J.
Médium: Artigo
Jazyk:Inglês
Vydáno: Nature Publishing Group UK 2019
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6365638/
https://ncbi.nlm.nih.gov/pubmed/30728395
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-37672-7
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