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Correlative cryo super-resolution light and electron microscopy on mammalian cells using fluorescent proteins

Sample fixation by vitrification is critical for the optimal structural preservation of biomolecules and subsequent high-resolution imaging by cryo-correlative light and electron microscopy (cryoCLEM). There is a large resolution gap between cryo fluorescence microscopy (cryoFLM), ~400-nm, and the s...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Tuijtel, Maarten W., Koster, Abraham J., Jakobs, Stefan, Faas, Frank G. A., Sharp, Thomas H.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6362030/
https://ncbi.nlm.nih.gov/pubmed/30718653
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-37728-8
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