In-plate recapturing of a dual-tagged recombinant Fasciola antigen (FhLAP) by a monoclonal antibody (US9) prevents non-specific binding in ELISA

Recombinant proteins expressed in E. coli are frequently purified by immobilized metal affinity chromatography (IMAC). By means of this technique, tagged proteins containing a polyhistidine sequence can be obtained up to 95% pure in a single step, but some host proteins also bind with great affinity...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Orbegozo-Medina, Ricardo A., Martínez-Sernández, Victoria, Perteguer, María J., Hernández-González, Ana, Mezo, Mercedes, González-Warleta, Marta, Romarís, Fernanda, Paniagua, Esperanza, Gárate, Teresa, Ubeira, Florencio M.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2019
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6358068/
https://ncbi.nlm.nih.gov/pubmed/30707711
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0211035
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