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Single tube allele specific PCR: a low cost technique for molecular screening of sickle cell anaemia in Nigeria

BACKGROUND: Sickle cell anaemia (SCA) is a hereditary blood disorder caused by a single mutation in the haemoglobin gene. The disease burden of SCA is highest in Nigeria. The allele specific polymerase chain reaction (ASPCR) method is applicable for the direct detection of known single nucleotide po...

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Detalhes bibliográficos
Publicado no:Afr Health Sci
Main Authors: Toye, Emuejevoke T, van Marle, Guido, Hutchins, Wendy, Abgabiaje, Olayinka, Okpuzor, Joy
Formato: Artigo
Idioma:Inglês
Publicado em: Makerere Medical School 2018
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6354853/
https://ncbi.nlm.nih.gov/pubmed/30766565
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4314/ahs.v18i4.20
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