A new full-length circular DNA sequencing method for viral-sized genomes reveals that RNAi transgenic plants provoke a shift in geminivirus populations in the field

We present a new method, CIDER-Seq (Circular DNA Enrichment sequencing) for the unbiased enrichment and long-read sequencing of viral-sized circular DNA molecules. We used CIDER-Seq to produce single-read full-length virus genomes for the first time. CIDER-Seq combines PCR-free virus enrichment with...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Mehta, Devang, Hirsch-Hoffmann, Matthias, Were, Mariam, Patrignani, Andrea, Zaidi, Syed Shan-e-Ali, Were, Hassan, Gruissem, Wilhelm, Vanderschuren, Hervé
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2019
Assuntos:
Methods Online
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6344846/
https://ncbi.nlm.nih.gov/pubmed/30357413
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gky914
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