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A Bacterial Expression Vector Archive (BEVA) for Flexible Modular Assembly of Golden Gate-Compatible Vectors

We present a Bacterial Expression Vector Archive (BEVA) for the modular assembly of bacterial vectors compatible with both traditional and Golden Gate cloning, utilizing the Type IIS restriction enzyme Esp3I, and have demonstrated its use for Golden Gate cloning in Escherichia coli. Ideal for synthe...

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Detalhes bibliográficos
Publicado no:Front Microbiol
Main Authors: Geddes, Barney A., Mendoza-Suárez, Marcela A., Poole, Philip S.
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6339899/
https://ncbi.nlm.nih.gov/pubmed/30692983
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2018.03345
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