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A Bacterial Expression Vector Archive (BEVA) for Flexible Modular Assembly of Golden Gate-Compatible Vectors

We present a Bacterial Expression Vector Archive (BEVA) for the modular assembly of bacterial vectors compatible with both traditional and Golden Gate cloning, utilizing the Type IIS restriction enzyme Esp3I, and have demonstrated its use for Golden Gate cloning in Escherichia coli. Ideal for synthe...

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Détails bibliographiques
Publié dans:Front Microbiol
Auteurs principaux: Geddes, Barney A., Mendoza-Suárez, Marcela A., Poole, Philip S.
Format: Artigo
Langue:Inglês
Publié: Frontiers Media S.A. 2019
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Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC6339899/
https://ncbi.nlm.nih.gov/pubmed/30692983
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2018.03345
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