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Combining LOPIT with differential ultracentrifugation for high-resolution spatial proteomics

The study of protein localisation has greatly benefited from high-throughput methods utilising cellular fractionation and proteomic profiling. Hyperplexed Localisation of Organelle Proteins by Isotope Tagging (hyperLOPIT) is a well-established method in this area. It achieves high-resolution separat...

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Dettagli Bibliografici
Pubblicato in:Nat Commun
Autori principali: Geladaki, Aikaterini, Kočevar Britovšek, Nina, Breckels, Lisa M., Smith, Tom S., Vennard, Owen L., Mulvey, Claire M., Crook, Oliver M., Gatto, Laurent, Lilley, Kathryn S.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Nature Publishing Group UK 2019
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC6338729/
https://ncbi.nlm.nih.gov/pubmed/30659192
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-018-08191-w
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