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Quantitative identification of proteins that influence miRNA biogenesis by RNA pull-down–SILAC mass spectrometry (RP–SMS)
RNA-binding proteins mediate and control gene expression. As some examples, they regulate pre-mRNA synthesis and processing; mRNA localisation, translation and decay; and microRNA (miRNA) biogenesis and function. Here, we present a detailed protocol for RNA pull-down coupled to stable isotope labell...
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| Publicat a: | Methods |
|---|---|
| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Academic Press
2019
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6335501/ https://ncbi.nlm.nih.gov/pubmed/29890283 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2018.06.006 |
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