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CRISPR-Cap: multiplexed double-stranded DNA enrichment based on the CRISPR system

Existing methods to enrich target regions of genomic DNA based on PCR, hybridization capture, or molecular inversion probes have various drawbacks, including long experiment times and low throughput and/or enrichment quality. We developed CRISPR-Cap, a simple and scalable CRISPR-based method to enri...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Lee, Jeewon, Lim, Hyeonseob, Jang, Hoon, Hwang, Byungjin, Lee, Joon Ho, Cho, Junhyuk, Lee, Ji Hyun, Bang, Duhee
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6326800/
https://ncbi.nlm.nih.gov/pubmed/30215766
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gky820
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