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Transgenerational CRISPR-Cas9 Activity Facilitates Multiplex Gene Editing in Allopolyploid Wheat

The CRISPR-Cas9-based multiplexed gene editing (MGE) provides a powerful method to modify multiple genomic regions simultaneously controlling different agronomic traits in crops. We applied the MGE construct built by combining the tandemly arrayed tRNA–gRNA units to generate heritable mutations in t...

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Detalhes bibliográficos
Publicado no:CRISPR J
Main Authors: Wang, Wei, Pan, Qianli, He, Fei, Akhunova, Alina, Chao, Shiaoman, Trick, Harold, Akhunov, Eduard
Formato: Artigo
Idioma:Inglês
Publicado em: Mary Ann Liebert, Inc. 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6319321/
https://ncbi.nlm.nih.gov/pubmed/30627700
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/crispr.2017.0010
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