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Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy

Many important signaling receptors are internalized through the well-studied process of clathrin-mediated endocytosis (CME). Traditional cell biological assays, measuring global changes in endocytosis, have identified over 30 known components participating in CME, and biochemical studies have genera...

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Detalles Bibliográficos
Publicado en:J Vis Exp
Main Authors: Soohoo, Amanda L., Bowersox, Shanna L., Puthenveedu, Manojkumar A.
Formato: Artigo
Idioma:Inglês
Publicado: 2014
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC6317842/
https://ncbi.nlm.nih.gov/pubmed/25350161
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51805
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