Rapid, Reproducible, Quantifiable NMR Metabolomics: Methanol and Methanol: Chloroform Precipitation for Removal of Macromolecules in Serum and Whole Blood

Background: Though blood is an excellent biofluid for metabolomics, proteins and lipids present in blood can interfere with 1d-(1)H NMR spectra and disrupt quantification of metabolites. Here, we present effective macromolecule removal strategies for serum and whole blood (WB) samples. Methods: A va...

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Detalhes bibliográficos
Publicado no:Metabolites
Main Authors: McHugh, Cora E., Flott, Thomas L., Schooff, Casey R., Smiley, Zyad, Puskarich, Michael A., Myers, Daniel D., Younger, John G., Jones, Alan E., Stringer, Kathleen A.
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI 2018
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6316042/
https://ncbi.nlm.nih.gov/pubmed/30558115
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/metabo8040093
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