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Multiphoton Imaging of Collagen, Elastin and Calcification in Intact Soft Tissue Samples

Multiphoton induced second harmonic generation and two photon excitation enables imaging of collagen and elastin fibers at micron level resolution to depths of hundreds of microns, without exogenous stains. These attributes can be leveraged for quantitative analysis of the 3D architecture of collage...

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Detalhes bibliográficos
Publicado no:Curr Protoc Cytom
Main Authors: Gade, Piyusha S., Robertson, Anne M., Chuang, Chih-Yuan
Formato: Artigo
Idioma:Inglês
Publicado em: 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6314890/
https://ncbi.nlm.nih.gov/pubmed/30379412
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpcy.51
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