Optimized ChIP-seq method facilitates transcription factor profiling in human tumors

Chromatin immunoprecipitation (ChIP)-seq analyses of transcription factors in clinical specimens are challenging due to the technical limitations and low quantities of starting material, often resulting in low enrichments and poor signal-to-noise ratio. Here, we present an optimized protocol for tra...

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Vydáno v:Life Sci Alliance
Hlavní autoři: Singh, Abhishek A, Schuurman, Karianne, Nevedomskaya, Ekaterina, Stelloo, Suzan, Linder, Simon, Droog, Marjolein, Kim, Yongsoo, Sanders, Joyce, van der Poel, Henk, Bergman, Andries M, Wessels, Lodewyk FA, Zwart, Wilbert
Médium: Artigo
Jazyk:Inglês
Vydáno: Life Science Alliance LLC 2018
Témata:
Methods
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6311467/
https://ncbi.nlm.nih.gov/pubmed/30620009
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.26508/lsa.201800115
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