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Relative Quantitation of Polymerized Actin in Suspension Cells by Flow Cytometry

The amount of polymerized actin within a cell can vary widely due to natural processes and/or experimentally induced perturbations. We routinely use this protocol to measure relative polymerized actin content between cell populations by staining cells in suspension with fluorescent phalloidin, then...

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Bibliografiske detaljer
Udgivet i:Bio Protoc
Main Authors: Kakley, Mallory R., Velle, Katrina B., Fritz-Laylin, Lillian K.
Format: Artigo
Sprog:Inglês
Udgivet: Bio-Protocol 2018
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6301187/
https://ncbi.nlm.nih.gov/pubmed/30581891
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3094
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